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Comparison of agglutinating and neutralizing antibodies to serovar hardjo in sows immunized with two commercial whole culture polivalent anti-leptospira bacterins BJM
Soto,Francisco Rafael Martins; Pinheiro,Sônia Regina; Morais,Zenaide Maria; Gonçales,Amane Paldês; Azevedo,Sérgio Santos de; Bernardi,Fernanda; Camargo,Sebastião Rodrigues; Vasconcellos,Silvio Arruda.
It was performed the comparison of the intensity and duration of agglutinating and neutralizing antibodies to serovar Hardjo in swines vaccinated with two commercial anti-leptospira bacterins. Sows no reactive to 24 Leptospira sp serovars in the microscopic agglutination test (MAT) were divided in three groups: Group A (n=08): received two vaccine A doses with 30 days interval, Group B (n=08) two vaccine B doses with 30 days interval and Group C (n=08): control no vaccinated against leptospirosis.Blood samples were collected each 30 days during six months following the first vaccination. The sera were tested by MAT and growth inhibition test (GIT) to serovar Hardjo in order to evaluate respectively agglutinating and neutralizing antibodies. It was found...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Swine; Leptospirosis; Immunity; Bacterines; Vaccination.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300013
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Cross-protection between experimental anti-leptospirosis bacterins BJM
Dib,Cristina Corsi; Gonçales,Amane Paldês; Morais,Zenaide Maria de; Souza,Gisele Oliveira de; Miraglia,Fabiana; Abreu,Patricia Antonia Estima; Vasconcellos,Silvio Arruda.
We investigated the existence of cross-protection between two anti-leptospirosis monovalent experimental bacterins produced with two strains of Leptospira serogroup Pomona: Fromm strain of serovar Kennewicky, isolated from pigs in the United States, and strain GR6 of serovar Pomona isolated from pigs in Brazil. Both were added of aluminum hydroxide as an adjuvant. Experimental bacterins were tested with the hamster potency test in order to assess protection provided against the disease and against the establishment of kidney infection. Controls were polyvalent commercial vaccine produced with Leptospira strains isolated outside Brazil, which included a representative of Pomona serovar, or Sorensen solution added of aluminum hydroxide adjuvant. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pomona; Hamsters; Bacterin; Cross-protection.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300042
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Efficacy of leptospiral commercial vaccines on the protection against an autochtonous strain recovered in Brazil BJM
Sonada,Rafael Bazaglia; Azevedo,Sérgio Santos de; Soto,Francisco Rafael Martins; Costa,Diego Figueiredo da; Morais,Zenaide Maria de; Souza,Gisele Oliveira de; Gonçales,Amane Paldês; Miraglia,Fabiana; Vasconcellos,Sílvio Arruda.
Abstract In swine and bovines, leptospirosis prevention and control is carried out via vaccination of susceptible animals using bacterins. However, the efficiency of leptospirosis vaccines has been questioned. This work aimed to investigate the potency of five leptospirosis vaccines sold commercially in Brazil, challenging the animals with one autochthonous strain of Leptospira, Canicola serovar, denoted LO4, isolated from swine. The standard protocol was followed, and renal carriers of Leptospira were identified among the surviving animals by culture and PCR. Of the five vaccines tested, only two proved effective. None of the surviving animals was positive by culture; however, one animal was positive by PCR. Three of the five vaccines sold commercially in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leptospirosis; Vaccination; Production animals; Control.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200347
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First isolation of leptospires from dairy goats in Brazil BJM
Lilenbaum,Walter; Morais,Zenaide M.; Gonçales,Amane Paldês; Souza,Gisele O. de; Richtzenhain,Leonardo; Vasconcellos,Silvio A..
Leptospires have never been recovered from goats in Brazil. Serum samples were obtained from 248 goats from Rio de Janeiro and from the seroreactive animals, urine samples were collected and processed for Leptospira isolation. A total of 52 positive reactions were observed, corresponding to 20.9% of the samples. The most prevalent reactions were to serovars Hardjo (36.5%), Shermani (30.8%), Icterohaemorrhagiae (9.6%), Grippotyphosa (9.6%), Autumnalis (5.8%), Castellonis (3.8%) and Bratislava (3.8%). Two strains of Leptospira sp. were isolated, both in the same region, but from different flocks. Presumptive identification based on serologic methods suggests those strains to be from Grippotyphosa serogroup.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Goats; Leptospirosis; Leptospira sp.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300023
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